Abstract: The study's focus is on the distribution of oxygen and hydrogen isotopes in the ice cores of frost blisters in the Chara River valley, Transbaikalia. The Kodar and Udokan mountain ranges surround the Chara Depression. Cryogenic (frosty) heaving is common in high humidity areas with loamy, clay, and peaty sediments. In summer 2023, the authors described two seasonal frost blisters in swampy, forested depressions on the periphery of the Charsky Sands (in the so-called thermosuffusion funnels). Oxygen and hydrogen isotopic compositions of oxygen  (δ18O) and hydrogen (δ2H) in blister ice were analyzed. It has been established that seasonal frost blisters are formed mainly due to downward freezing of the water-saturated active layer sediments, but in some cases, ice was formed as well due to upward freezing from the lower boundary of the active layer. The vertical distribution of the isotope values, as well as the δ2H-δ18O ratio in ice, indicates a relatively rapid freezing of water in closed system conditions during one cycle of freezing. In the ice layers sampled horizontally, very similar isotopic composition values were obtained (for example, in blister ice 1, δ18О values ranged from –17.51 to –17.32‰), which indicates consistent horizontal freezing of ice layers. The slopes of the δ2H-δ18О ratio lines for blister ice are 5.82 (blister ice 1) and 5.95 (blister ice 2). A decrease of the δ2H and δ18О values of blister ice on frost mounds with depth and a slope of the δ2H-δ18O ratio line less than 8–7.3 indicates water freezing under closed system conditions during one cycle without moisture inflow. The distribution of isotopic values in the blister ice of seasonal frost mound 2 may indicate simultaneous bilateral freezing of the water volume.